Formation of enzyme-substrate complex (ES) is the first step in catalysed reactions. Describe the other steps till the formation of product.

Step 1:

It involves the formation of E-S substrate complex, formed when enzyme binds to substrate at active site.

Step2: Transient phase

• The structure of substrate changes, during this very high energy of activation is involved.

Step3: Formation phase

• Finally the product gets formed.

• Enzyme gets free to bind with new substrate.

• Potential energy of the system declines.