Note: In the following questions a statement of assertion followed by a statement of reason is given. Choose the correct answer out of the following choices.

Assertion : Non-competitive inhibitor inhibits the catalytic activity of enzyme by binding with its active site.

Reason : Non-competitive inhibitor changes the shape of the active site in such a way that substrate can’t recognise it.

• Assertion statement is incorrect because, non-competitive inhibitors only bind to a different site of any enzyme which is generally called the allosteric site .

• Reason statement is correct because, by binding at the allosteric site the non-competitive inhibitors changes the shape of the active site of the enzyme in such a way that the substrate will not be able to identify it.

• Therefore, enzyme-substrate bonding is not possible.