For the same objective, find the ratio of the least separation between two points to be distinguished by a microscope for light of 5000 and electrons accelerated through 100V used as the illuminating substance.
Least separation is given by … (i) , where λ = wavelength, β = angle subtended by the objective at the object.
For light of wavelength 5000 , .. (ii)
For electron accelerated through 100 V, the De- Broglie wavelength is given by , where h = Planck’s constant = 6.63 x 10-34 kgm2s-1, p = momentum.
Now, kinetic energy of electron is given by , where e = electronic charge, V = voltage through which electrons are accelerated, p = momentum, m = mass of electron. Therefore,
.
Hence,
Hence, least separation in this case is given by … (iii)
Hence, required ratio (Ans)