Q7 of 32 Page 204

Discuss in detail how RNA can be used to silence specific genes.

• A nematode called the Meliodygyne incognitia is known to cause root knots in tobacco plant.


• Scientists developed a method called RNAi to kill this nematode.


• A host specific double stranded DNA was introduced, such that it produced both sense and antisense sequences.


• Both these sense ad antisense sequences undergo transcription and form two complementary RNA strands.


• Both the strands combine to form double helix.


• Which is chopped off, hence the gene expression of nematode inactivates.


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