Imagine that student A studied the importance of certain factors in photosynthesis. He took a potted plant and kept it in dark for over 24 hours. In the early hours of the next morning, he covered one of the leaves with dark paper in the centre only. Then he placed the plant in sunlight for a few hours and tested the leaf which was covered with black paper for starch.
a) What aspect of photosynthesis was being investigated?
b) Why was the plant kept in the dark before the experiment?
c) How will you prove that starch is present in the leaves?
d) What are the other raw materials for photosynthesis?
a. Sunlight is required for photosynthesis. This experiment shows that sunlight is an important factor that is required for photosynthesis. Because in absence of sunlight glucose cannot be synthesized by the leaves of the plant.
b. The plant was kept in dark for 24-48 hours to destarch the plant so that whole stored starch would be used up by the plant and it will not interfere on the experiments of photosynthesis.
c. First the leaf is placed in boiling water followed by boiling the leaf in methylated spirit which removes chlorophyll. Then iodine solution is poured over leaf. The blue-black colour will indicate the presence of starch whereas brown colour will indicate absence of starch.
d. Carbon dioxide, water and chlorophyll are required for photosynthesis.
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