Q2 of 14 Page 104

Answer the following:

Explain the method of testing a leaf for starch.

Aim: To test the presence of starch in a leaf.


Material required:


Electric water bath or hot plate, 3 beakers of 250 ml, boiling tube, forceps, test tube holder, leaf to be tested ( leaf of China rose will be good), 90 % ethanol and iodine solution.


Procedure:


● Remove a green leaf from a plant that has been exposed to sunlight for a few hours.


● Fill three 250ml beakers half with water. Heat the water until it boils. Keep the water at boiling point. Put the leaf in the boiling water using forceps.


● Boil for 2 minutes.


● Put the boiled leaf in a boiling tube containing 90% ethanol.


● Put the boiling tube in hot water and boil for 10 minutes to decolorize leaf.


● Remove the leaf and wash cold water.


● Add a few drops of iodine solution to the leaf and observe.


After iodine solution was added...




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