Name the host plant and its part that Meloidogyne incognita infects. Explain the role of Agrobacterium in the production of ds-RNA in the host plant.
Tobacco is the host plant and roots of that infected by Meloidegyne incognitia.
• In the host plant, nematode-specific genes were introduced by using Agrobacterium as a vector.
• In the host cell, both sense and antisense RNA is produced by the introduction of DNA.
• These two RNAs formed an inactive double-stranded RNA (dsRNA) that silenced the specific mRNA of the nematode, that the process called RNA interference (RNAi).
• This results in the protection of the transgenic plant from the parasites which arenot able to survive in the transgenic host.
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