Q4 of 19 Page 125

If the sequence of the coding strand in a transcription unit is written as follows: 5'-ATGCATGCATGCATGCATGCATGCATGC-3'

Write down the sequence of mRNA.

The sequence of mRNA will be 5'-AUGCAUGCAUGCAUGCAUGCAUGCAUGC-3'.


Explanation: In a RNA strand thymine is replaced with uracil. The coding strand of mRNA is the same as that of DNA with the only difference being uracil instead of thymine. If the coding strand is 5'-ATGCATGCATGCATGCATGCATGCATGC-3' then the mRNA sequence will be 5'-AUGCAUGCAUGCAUGCAUGCAUGCAUGC-3'.


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2

If a double stranded DNA has 20 per cent of cytosine, calculate the per cent of adenine in the DNA.

 3

If the sequence of one strand of DNA is written as follows:

5'-ATGCATGCATGCATGCATGCATGCATGC-3'


Write down the sequence of complementary strand in 5' 3' direction.

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Which property of DNA double helix led Watson and Crick to hypothesise semi-conservative mode of DNA replication? Explain.

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Depending upon the chemical nature of the template (DNA or RNA) and the nature of nucleic acids synthesized from it (DNA or RNA), list the types of nucleic acid polymerases.