PCR requires very high-temperature conditions where most of the enzymes get denatured. How was this problem resolved in a PCR?
PCR or Polymerase Chain Reaction is a beneficial technique through which multiple copies of new DNA strands are made from the desired strand. The new strands of DNA are made from the existing or template strand with the help of an enzyme called DNA polymerase. In this process, high temperature is required to carry out the processes. Such high temperatures might harm the enzymes as they will get denatured. To solve such temperature related problems, the type of DNA polymerase used is heat tolerant and is called Taq polymerase. It is extracted and isolated from a bacterium called Thermus aquaticus. This bacteria itself can tolerate high temperatures as it lives in hot springs and hydrothermal vents. By staying in such hot environments its DNA polymerase has become stable towards high temperatures such as 70°C, at which normal human or other bacterium DNA polymerase.
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