Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool.

The 5 tools for rDNA technology are as follows

a) Restriction endonuclease - It cuts the DNA sequence at specific positions.

b) Vector - It is the most important tool for the rDNA technology vector contains site for the insertion of gene of interest called the origin of replication.

c) DNA ligase - It facilitates the joining of desired DNA with vector DNA.

d) Polymerase - The enzyme makes multiple copies of rDNA.

e) Host cell - The cell which takes up rDNA & change itself to transformant.