Q21 of 42 Page 1

(a) Mention the importance of gel-electrophoresis in biotechnology.

(b) Explain the process of this technique.



a) The gel electrophoresis technique is very important in the field of biotechnology. It helps in cutting of desired DNA segment and the separation of the DNA fragments for biotechnology experiments.


b)


• DNA being negatively charged moves towards anode, under electric field through a matrix or an agarose medium.


• DNA fragments separate according to their size.


• Smaller the size farther they tend to move


• The separated DNA fragments are stained with ethidium bromide and visualized by exposing them to UV radiation and are cut.


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