A biotechnologist wanted to create a colony of E.coli possessing the plasmid pBR322, sensitive to Tetracycline. Which one of the following restriction sites would he use to ligate a foreign DNA?

• The biotechnologist can ligate the foreign DNA at the Sal I of Tetracycline resistance gene in pBR322 vector.

• Since the Tetracycline resistance gene has 2 restriction sites, namely BamH I and Sal I, the foreign DNA can be ligated at any of these 2 sites.

Option B is incorrect as Pvu I is a restriction site present in the Ampicillin resistance gene.

Option C is incorrect as EcoRI is present within the promoter of Ampicillin resistance gene.

Option D is incorrect as Hind III is present within the promoter of Tetracycline.