Q25 of 30 Page 1

A vector is engineered with three features which facilitate its cloning within the host cell. List the three features and explain each one of them.

The three features which facilitate the cloning of a vector within the host cell:-


a) Origin of replication (ori) – This is a sequence from where replication starts and any piece of DNA when linked to this sequence can be made to replicate within the host cells.


b) Selectable marker – They help in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.


c) Cloning sites – In order to link the alien DNA, the vector needs to have a very few, preferably single, recognition sites for the commonly used restriction enzymes.


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