Q20 of 31 Page 1

CryIAb is introduced in a plant to prevent infestation by corn borer.

(a) What is the resultant plant referred as?


(b) Summarize the action of the gene introduced

(a) Bt corn. It is a type of genetically modified crop. It has been genetically modified through the addition of a small amount of genetic material from other organisms through molecular techniques.


(b) CryIAb gene codes for crystal protein. The Bt toxin protein exists as an inactive protein. On ingestion by an insect, the Bt toxin gets converted into an active form due to the alkaline pH of the gut which solubilizes the crystal. The activated toxin binds to the surface of mid gut and creates pores which cause death of the insect.


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19

A doctor prescribed morphine as a sedative and pain killer to your cousin who had undergone a surgery. Even after recovery, he indiscriminately took the medicines and later craved for the same. What do you conclude about his condition? What measures will you suggest to him to overcome this problem? Briefly explain any two.

 20

Given below is the diagram of agarose gel kept under UV light:


(a) Mention the positive and negative terminals.


(b) What is the charge carried by DNA molecule and how does it help in its separation?


(c) How are the separated DNA fragments finally isolated?

 21

(a) In pBR322, foreign DNA has to be introduced in tetR region. From the restriction enzymes given below, which one should be used and why:

Pvu I, EcoR I, BamH I


(b) Give reasons, why the other two enzymes cannot be used.

 22

The graph given below shows the distribution of biomes:


(a) What do the ‘X’ and ‘Y’ axis represent?


(b) Identify the ‘grassland’ and ‘coniferous forest’ biomes, from the above figure.


(c) Why is ‘F’ located at the given position in the graph?