Q21 of 31 Page 1

(a) In pBR322, foreign DNA has to be introduced in tetR region. From the restriction enzymes given below, which one should be used and why:

Pvu I, EcoR I, BamH I


(b) Give reasons, why the other two enzymes cannot be used.

(a) BamH I should be used as restriction site for this enzyme which is present in tetR region.


(b) Pvu I cannot be used, as restriction site for this enzyme is present in ampR region, not in tetR.


EcoR I cannot be used, as restriction site for this enzyme is not present in selectable marker tetR.


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20

Given below is the diagram of agarose gel kept under UV light:


(a) Mention the positive and negative terminals.


(b) What is the charge carried by DNA molecule and how does it help in its separation?


(c) How are the separated DNA fragments finally isolated?

 20

CryIAb is introduced in a plant to prevent infestation by corn borer.

(a) What is the resultant plant referred as?


(b) Summarize the action of the gene introduced

 22

The graph given below shows the distribution of biomes:


(a) What do the ‘X’ and ‘Y’ axis represent?


(b) Identify the ‘grassland’ and ‘coniferous forest’ biomes, from the above figure.


(c) Why is ‘F’ located at the given position in the graph?

 23

A son persuades his father to replace his old mobile phone with the latest model launched in the market. He also shares the latest features it has and explains how it can be of a help to him in the modern technological world. Father is reluctant in buying a new one and tries to explain about its environmental impact. How do you think, the biologist father would try to convince his son? Justify the arguments of father and son both, by mentioning positive aspects of the behavior displayed by both of them in the situation concerned (three each).