(a) Given below is a single stranded DNA molecule. Frame and label its sense and antisense RNA molecule. 5’ ATGGGGCTC 3’ sense

(b) How the RNA molecules made from above DNA strand help in silencing of the specific RNA molecules?

When someone buys packets of cigarettes, cannot miss the statutory warning that is present on the packing which warns against smoking and says how it is injurious to health. Yet, smoking is very prevalent in our society, both among young and old. Advise the adolescents about the importance of avoiding smoking. (Mention any six points.)

Biotechnology has helped farmers to get pest resistant cotton crops. Explain the technique adopted along with its mode of action. (Mention six points)

OR

Draw the figure of vector pBR322 and label the following: Origin of replication, Ampicillin resistance site, Tetracycline resistance site, Bam H1 restriction site.

Rajesh was doing gel electrophoresis to purify DNA fragments. Given below is the sketch of the observations of the experiment performed by him.

(a) At which end he would have loaded the samples and where?

(b) Analyse the reason for different positions taken up by the DNA bands.

(c) Elaborate the step he would have followed to visualize DNA bands.

Mohit and Sumit want to buy a new car for their company. Mohit insisted on buying a CNG car with a better mileage but Sumit insisted on buying a diesel version of a high end car with better music system and A.C but relatively low mileage.

(a) Being a responsible citizen of Delhi, how will Mohit convince Sumit about his decision in the wake of rising pollution levels?

(b) What qualities of personality are being exhibited by Mohit in doing so?

(c) Suggest two more measures which can help in reducing vehicular pollution.