Q10 of 29 Page 1

How are recombinant vectors created? Why is only one type of restriction endonuclease required for creating one recombinant vector?

To create recombinant vectors -


The vector DNA is cut at a particular restriction site using a restriction enzyme (to cut the desired DNA segment). The alien DNA is then linked with the plasmid DNA using an enzyme called ligase to form the recombinant vector.


Since a restriction enzyme recognises and cuts the DNA at a particular sequence is called recognition site, the same restriction enzyme is used for cutting the DNA segment from both the vector and the other source.


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The figure below shows the relative contribution of four green house gases to global warming:


(i) Identify the gases A and C.


(ii) Why are these four gases called the green house gases?