Q16 of 29 Page 1

Name the organism from where the thermostable DNA polymerase is isolated. State its role in genetic engineering.

The thermostable DNA polymerase is isolated from a Thermus aquaticus which remain active during the high temperature induced denaturation of double stranded DNA.


Its role in genetic engineering :-


i. Since it can withstand the high temperature, it is used in denaturation and separation of DNA strands during Polymerase Chain Reaction (PCR).


ii. It extends the primers using the nucleotides provided in the reaction and the genomic DNA as tempelate.


iii. Repeated amplification can be achieved by this enzyme. The amplified fragments, if desired can be used to ligate with a vector for further cloning.


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