The promoter site and the terminator site for transcription are located at:

•A promoter is a region of DNA where RNA polymerase binds to initiate transcription. It is present at the 5’ end of the transcription unit.

•A terminator is a sequence of DNA that causes RNA polymerase to terminate transcription. It is present at the 3’ end of the transcription unit.

•A transcription unit is the sequence between sites of initiation and termination by RNA polymerase; may include more than one gene.

•Upstream identifies sequences in the opposite direction from expression; for example, the bacterial promoter is upstream of the transcription unit, the initiation codon is upstream of the coding region.

•Downstream identifies sequences proceeding farther in the direction of expression; for example, the coding region is downstream of the initiation codon.