While analysing the DNA of an organism a total number of 5386 nucleotides were found out of which the proportion of different bases were: Adenine = 29%, Guanine = 17%, Cytosine = 32%, Thymine = 17%. Considering the Chargaff’s rule, it can be concluded that:

According to Chargaff’s rule of base pairing:

1) Adenine is equal to thymine. They are bonded by two hydrogen bonds.

2) Guanine is equal to cytosine. They are bonded by three hydrogen bonds.

3)The ratio of adenine to thymine and Guanine to Cytosine is equal to 1 which proves DNA is a double stranded structure.

4)In the above organism the DNA is not following the Chargaff’s rule hence it shows it is not a double stranded DNA but a single stranded DNA.