Q18 of 71 Page 41

Would it be appropriate to use DNA probes such as VNTR in DNA finger printing of a bacteriophage?

1. VNTR is the Variable Number of Tandem repeats. It is a chromosomal region. In which a small stretch of DNA sequence is repeated numerous times in a single location.


2. These VNTR produce a pattern of bands which is unique for each individual. These VNTR are used to identify criminals in forensic departments.


3. DNA fingerprinting is not an option for bacteriophage as it does not have repetitive sequencing portions like in VNTR’S so a pattern of bands would not be formed. Bacteriophagehas a small genome that has all the coding sequences.


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Now, sequencing of total genomes getting is getting less expensive day by the day. Soon it may be affordable for a common man to get his genome sequenced. What in your opinion could be the advantage and disadvantage of this development?

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During in vitro synthesis of DNA, a researcher used 2’, 3’ – dideoxy cytidine triphosphate as raw nucleotide in place of 2’-deoxy cytidine. What would be the consequence?

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What background information did Watson and Crick have made available for developing a model of DNA? What was their contribution?