Rearrange the following in the correct sequence to accomplish an important biotechnological reaction:

(a) In vitro synthesis of copies of DNA of interest

(b) Chemically synthesized oligonucleotides

(c) Enzyme DNA-polymerase

(d) Complementary region of DNA

(e) Genomic DNA template

(f) Nucleotides provided

(g) Primers

(h) Thermostable DNA-polymerase (from Thermus aquaticus)

(i) Denaturation of ds-DNA

Enumerate any six essentials of good, effective Dairy Farm Management Practices.

State the medicinal value and the bioactive molecules produced by Streptococcus, Monascus and Trichoderma.

OR

What are methanogens? How do they help to generate biogas?

Describe any three potential applications of genetically modified plants.

How did an American Company, Eli Lilly use the knowledge of r-DNA technology to produce human insulin?