Q18 of 50 Page 1

How are 'sticky ends’ formed on a DNA strand ? Why are they so called ?

Sticky ends are produced by the enzyme Restriction endonuclease.


• Restriction endonuclease produce staggered cuts in which the two strands of a DNA double helix ae cleaved at a different locations this generates a protruding (3’ or 5’) ends, that is one strand of the double helix extends some bases beyond the other.


• Due to palindromic nature of the target sites the two ends produce such kind of sticky ends or cohesive ends.


• These are callrd sticky ends because they form hydrogen bonds with their complementary cut counterparts.


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