Q18 of 50 Page 1

Write the role of 'Ori' and 'restriction’ site in a cloning vector pBR322.



Ori: It is a genetic sequence, which is the initiation site of replication of DNA. Any DNA fragment which is attached to this site starts replicating


Restriction site: It is the site where restriction enzymes such as HindIII, EcoRI, BamHI etc. are recognised.


More from this chapter

All 50 →
13

How can healthy potato plants be obtained from a desired potato variety which is viral infected ? Explain.

 15

What is Bio piracy? State the initiative taken by the Indian Parliament towards it.

 20

A cross between a normal couple resulted in a son who was haemophilic and a normal daughter. In course of time, when the daughter was married to a normal man, to their surprise, the grandson was also haemophilic.

(a) Represent this cross in the form of a pedigree chart. Give the genotypes of the daughter and her husband.


(b) Write the conclusion you draw of the inheritance pattern of this disease.


 22

Draw a labelled diagram of the sectional view of a human seminiferous tubule (six parts to be labelled)