Q6 of 44 Page 75

Which of the following is not required in the preparation of a recombinant DNA molecule?

•Restriction enzymes act as molecular scissors and cut the DNA into fragment.


•DNA ligase is used to construct rDNA, by joining fragment of DNA [gene of interest] with the DNA of genetic material of the vector.

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4

Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?

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'Restriction' in Restriction enzyme refers to:

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In agarose gel electrophoresis, DNA molecules are separated on the basis of their:

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The most important feature in a plasmid to serve as a vector in gene cloning experiment is: