Q2 of 44 Page 81

Describe the role of Agrobacterium tumefaciens in transforming a plant cell.

•Agrobacterium tumefaciens is known to cause infection in plants especially in dicots.


•The Ti plasmid of this bacterium induces tumour formation inside the plant cell.


•The DNA of Ti plasmid gets incorporated with the host cell DNA.


•Recombinant DNA dictates, to form the substances which are necessary for the growth of bacteria.


•However, the tumour causing gene has been deleted by molecular biologist in order to use it as a vector.


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11

Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.


 12

Name the regions marked A, B and C.


 1

For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chormogenic substrate. Give reasons.

 3

Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.