Q9 of 44 Page 78

Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?

•No, in absence of water biomolecules become dysfunctional.


•Water is involved in H-bonding hence it plays an important role in the maintenance of the structure of DNA and protein.


•If H-bond weakens or collapses, it results in denaturation of biomolecules.


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While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?

•


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