Q12 of 44 Page 75

Significance of 'heat shock' method in bacterial transformation is to facilitate:

•The host is rubbed by diavalent cation like Ca2+, this results in the increase in diameter of pores of bacterial host cell wall.


•The host and rDNA are than kept on ice, then heated at 420 C and again kept on ice.


•This treatment makes the cell wall competent and allow to uptake foreign DNA.

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Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?

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An antibiotic resistance gene in a vector usually helps in the selection of:

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The role of DNA ligase in the construction of a recombinant DNA molecule is:

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Which of the following bacteria is not a source of restriction endonuclease?