Q14 of 44 Page 75

Which of the following bacteria is not a source of restriction endonuclease?

•Agrobacterium tumificiean is genetically engineered to make it act as a vector.


•The pathogenic gene of Ti plasmid has been disarmed, which is known to induce tumour formation.

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Significance of 'heat shock' method in bacterial transformation is to facilitate:

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The role of DNA ligase in the construction of a recombinant DNA molecule is:

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Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?

 16

A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be: