Q6 of 44 Page 78

What is the significance of adding proteases at the time of isolation of genetic material (DNA).

•Proteases are the enzymes which digest the protein present inside the cell.


•Proteases ensure the isolation of DNA without its contamination with proteins.


•Moreover, protein causes interference during downstream processing.


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Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.

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What does ‘competent’ refer to in competent cells used in transformation experiments?

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While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?

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Name a recombinant vaccine that is currently being used in vaccination program.