Q3 of 44 Page 79

A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?

•It would not affect the constructed recombinant DNA.


•Recombinanat DNA being circular has no free ends, hence the endonuclease would not recognise the specific pallendromic nucleotide sequences and will not cut at specific positions.


More from this chapter

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1

What is meant by gene cloning?

 2

Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?

 4

Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?

 5

A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.