Q7 of 44 Page 79

A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?

•Absence of selectable marker would not affect the construction of recombinant DNA.


•But in absence of selectable marker, it would become difficult to distinguish between transformant and non transformant cell.


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A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.

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How does one visualise DNA on an agarose gel?

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A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?

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Describe the role of CaCl2 in the preparation of competent cells?