Q15 of 44 Page 75

Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?

Taq Polymerase is a thermostable DNA polymerase which can extent the primers by adding nucleotide even at a high temperature of 720C.

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13

The role of DNA ligase in the construction of a recombinant DNA molecule is:

 14

Which of the following bacteria is not a source of restriction endonuclease?

 16

A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be:

 17

Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?