Q4 of 44 Page 78

Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.

It would result in formation of multiple fragments, if a restriction enzyme would have more than one site of action.


More from this chapter

All 44 →
2

Would you choose an exonuclease while producing a recombinant DNA molecule?

 3

What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?

 5

What does ‘competent’ refer to in competent cells used in transformation experiments?

 6

What is the significance of adding proteases at the time of isolation of genetic material (DNA).