Q17 of 44 Page 75

Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?

•These include open system bioreactors which are opened at regular intervals and new media can be continuously added.


•The cell multiplies at faster rate, and the previous culture media is taken out while fresh media is added.

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15

Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?

 16

A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be:

 18

Who among the following was awarded the Nobel Prize for the development of PCR technique?

 19

Which of the following statements does not hold true for restriction enzyme?