Q1 of 44 Page 79

What is meant by gene cloning?

•Gene cloning involves the insertion of gene of interest within the DNA of a vector.


•These vectors are used to introduce the gene of interest inside the host cell.


•These host cell are known as transformants.


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Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?

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What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?

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Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?

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A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?