Q7 of 44 Page 78

While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?

•


If the denaturation step is missed, DNA strands would not separate from each other hence primer could not be added to the ends.

•It would disrupt the whole process of amplification.


More from this chapter

All 44 →
5

What does ‘competent’ refer to in competent cells used in transformation experiments?

 6

What is the significance of adding proteases at the time of isolation of genetic material (DNA).

 8

Name a recombinant vaccine that is currently being used in vaccination program.

 9

Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?