Q3 of 44 Page 81

Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.



Stirred bioreactors are large volume vessels used for large scale production whereas flasks in labs are used to obtain small volume of culture media.


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11

Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.


 12

Name the regions marked A, B and C.


 1

For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chormogenic substrate. Give reasons.

 2

Describe the role of Agrobacterium tumefaciens in transforming a plant cell.