Q11 of 44 Page 79

Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.


A- Denaturation, B- Annealing, C- extension


Denaturation- in this step double stranded DNA is denatured at high temperature of 940C for 15 seconds.


DNA strands separates, and act as template.


Annealing-Primers are added to anneal. This is done at 450 C using Mg2+ ions.


Extension- Taq polymerase is used to extend the primer by adding nucleotides at a temperature of 720C.


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Name the regions marked A, B and C.


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