Q2 of 44 Page 79

Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?

•Both approaches are correct.


•Wine maker uses yeast to produce ethanol (natural phenomenon).


•Whereas a molecular Biologist uses vector with high copy number to produce large amounts of antigens.


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10

What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?

 1

What is meant by gene cloning?

 3

A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?

 4

Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?