Q10 of 44 Page 75

Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?

In PCR during extention phase, taq polymerase is used to add nucleotide at a very high temperature of 720 C. Thermostable Taq polymerase remains stable and active even at such a high temperature.

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The most important feature in a plasmid to serve as a vector in gene cloning experiment is:

 9

While isolating DNA from bacteria, which of the following enzymes is not required?

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An antibiotic resistance gene in a vector usually helps in the selection of:

 12

Significance of 'heat shock' method in bacterial transformation is to facilitate: