Q2 of 44 Page 78

Would you choose an exonuclease while producing a recombinant DNA molecule?

•No, exonuclease removes nucleotide from the end of the DNA strands.


•If DNA is treated with exonuclease, no overhanging sticky ends would be produced. Hence rDNA could not be constructed.


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19

Which of the following statements does not hold true for restriction enzyme?

 1

How is copy number of the plasmid vector related to yield of recombinant protein?

 3

What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?

 4

Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.